DNA Extraction and Purification
Genelink Linear Acrylamide Solution (Linear polyacrylamide, LPA; 5mg/mL); 1 mL
Effective DNA and RNA precipitation with the use of monovalent salts and ethanol or isopropanol is dependent on its concentration. DNA and specifically RNA precipitation at a concentration of less than 50 ng/mL is usually not quantitative and the pellet is not clearly visible leading to unreliable recovery. Addition of linear acrylamide as a carrier/coprecipitant aids in quantitative recovery and specifically clear visibility pf the pellet. Linear acrylamide does not interfere with spectrophotometric readings, electrophoresis, and most molecular biology enzymatic applications including PCR. Linear acrylamide is prepared synthetically and is guaranteed free of all nucleic acids, DNase, RNase and proteases.
BIOSEARCH TECHNOLOGIES INC (Lucigen) MasterPure Complete DNA & RNA Purification Kit, 200 PurifQuickly purify high yields of high-molecular-weight genomic DNA, total cellular RNA or Total Nucleic Acid (TNA) with one kit.
The MasterPure™ Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types.
Macherey-Nagel MACHEREY-NAGEL NucleoBond HMW DNA Kit, High Integrity DNA Purification, Enzymatic Lysis Included, 20 Preps
MACHEREY-NAGEL NucleoBond HMW DNA kit is designed for the purification of high molecular weight DNA, ensuring high integrity suitable for long-read and Next Generation Sequencing applications. The kit utilizes anion-exchange chromatography and includes Proteinase K for gentle enzymatic lysis, minimizing DNA shearing. It is ideal for isolating DNA from cultured cells, tissue, and plant samples, making it a versatile choice for demanding research environments.
Sigma Aldrich Fine Chemicals Biosciences REDExtract-N-Amp(TM) Tissue PCR Kit | 100RXN
REDExtract-N-Amp(TM) Tissue PCR Kit | 100RXN
Zymo Research Corporation ZymoBIOMICS™ Microbial Community DNA Standard (2000 ng)
One of the major challenges in the emerging field of microbiomics is the bias and errors introduced in the complex workflows. Besides nucleic acid purification, bias also arises from sequencing library preparation and subsequent processes. The ZymoBIOMICS Microbial Community DNA Standard is designed to assess bias, errors and other artifacts after the step of nucleic acid purification. This DNA standard is created by pooling DNA extracted from pure cultures. It has an accurately defined composition, negligible impurities (0.01%), and contains genomes of a wide range of GC content (15% - 85%). This DNA standard is designed to have the same microbial composition with the cellular version, the ZymoBIOMICS Microbial Community Standard, so that they can be more powerful when working in tandem.
New England Biolabs, Inc. NEBNext dsDNA Fragmentase – 250 reactions
NEBNext dsDNA Fragmentase is an enzyme-based reagent that shears DNA to produce fragments of the desired sizes in a time-dependent manner, for next generation sequencing library preparation protocols Note: NEBNext dsDNA Fragmentase is not part of the NEBNext Ultra II FS (Fragmentation System) reagents.
Research Products International Corp DNA Wash Buffer, 6 ml
DNA Wash Buffer (concentrate) is designed to use as a column wash to remove salts and other contaminants prior to DNA elution. This ensures high purity DNA to be recovered. Ethanol must be added to the concentrate prior to use. Instructions for each DNA Wash Buffer (concentrate) size is listed on the bottle and within the Zymo Research protocols it is used in.
Research Products International Corp DNA Clean & Concentrator-100 w/Zymo Spin V, 25 Preps
The DNA Clean & Concentrator-100 (DCC-100) is designed for the rapid desalting and purification of up to 100 µg of high quality DNA from PCR, large format restriction endonuclease digestions, or cell-free lysates. Eluted DNA is suitable for nucleotide sequencing, array analysis, PCR, nucleotide blotting, restriction endonuclease digestion procedures, as well as many other downstream applications requiring high quality DNA. The entire DNA purification/concentration procedure typically takes less than 20 minutes and can be performed using a syringe, centrifuge or vacuum source together with a microcentrifuge.
Simple, rapid recovery of ultra-pure DNA from PCR, endonuclease digestions, and cell-free DNA preps., etc.
Unique column construction allows sample loading and washing to be performed using a centrifuge, microcentrifuge, vacuum source, or syringe.
Research Products International Corp Zymo Quick-DNA/RNA MagBead Kit, 96 Preps
The Quick-DNA/RNA MagBead Kit provides a high-throughput, magnetic-bead based purification of both high-quality DNA and total RNA (including small RNAs) from the same starting sample. The provided DNA/RNA Shield inactivates infectious agent and is ideal for sample storage at ambient temperatures. The extraction method has the option to recover total nucleic acids in one elution or DNA & RNA in separate elutions without the use of phenol. It is eluted into ≥50 µl of DNase/RNase-free water and is ready for any downstream application including RT-PCR, sequencing, etc.
Quick & Easy: Extract DNA and RNA from any sample including cells, solid tissue, whole blood, biological liquids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), etc.
Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, arrays, etc. DNase I included (Plus kit and MagBead only)
Research Products International Corp ZR Plasmid Miniprep-Classic Kit (400 Preps)
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
Research Products International Corp Quick-DNA MiniPrep w/Zymo-Spin IIC Columns, Capped, 50 Preps
The Quick-DNA Kits are ideal DNA isolation kits for easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples.
Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
